Extraction & qPCR Manuals and Literature

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Manuals

See your Technical Leader for clarification regarding specific manuals to equipment in your laboratory.

  1. Read your laboratory's SOPs regarding maintenance, calibration (if applicable), and cleaning of DNA laboratory equipment.
  2. Microcon/Centricon® User Manual
  3. AutoMate Express™ Instrument User Guide,  Life Technologies Corporation, pub. 4441982 RevD
  4. PrepFiler® and PrepFiler® BTA Forensic DNA Extraction Kits Quick Reference,  Applied Biosystems, 4468126 Rev. B
  5. PrepFiler Express™ and PrepFiler Express BTA™ Forensic DNA Extraction Kits User Guide,  Applied Biosystems, 4442699 Rev. B
Literature
  1. The Extraction, Purification and Quantification of DNA
    Baechtel, F. S. 1989. The extraction, purification and quantification of DNA. In Proceedings of the International Symposium on the Forensic Aspects of DNA Analysis, 25–28. Washington, D.C.: U.S. Government Printing Office.
  2. The Modified Method of Two-Step Differential Extraction of Sperm and Vaginal Epithelial Cell DNA from Vaginal Fluid Mixed with Semen
    Yoshida, K., K. Sekiguchi, N. Mizuno, K. Kasai, I. Sakai, H. Sato, and S.Seta.  1995. The modified method oftwo-step differential extraction of sperm and vaginal epithelial cell DNA from vaginal fluid mixed with semen. Forensic Sci Int 72(1): 25–33.
  3. DNA Extraction Strategies for Amplified Fragment Length Polymorphism Analysis, Comey, C. T. 1994.  DNA extraction strategies for amplified fragment length polymorphism analysis. JForensic Sci 39 (5): 1254–69.
  4. QIAamp Spin Columns as a Method of DNA Isolation for Forensic Casework
    Greenspoon, S. A., M. A. Scarpetta, M. L. Drayton, and S. A. Turek.  1998. QIAamp spin columns as a method of DNA isolation for forensic casework.  J Forensic Sci 43 (5): 1024–30
  5. Development of a Simulation Model to Assess the Impact of Contamination in Casework Using STRs
    Gill, P., and A. Kirkham.  2004. Development of a simulation model to assess the impact of contamination in casework using STRs. J Forensic Sci 49 (3): 485–91.
  6. A Systematic Analysis of PCR Contamination
    Scherczinger, C. A., C. Ladd, M. T. Bourke, M. S. Adamowicz, P. M. Johannes, R. Scherczinger, T. Beesley, and H. C. Lee.  1999. A systematic analysis of PCR contamination. J Forensic Sci 44 (5): 1042–5.
  7. Quality Assurance Standards for Forensic DNA Testing Laboratories. http://www.fbi.gov/about-us/lab/biometric-analysis/codis/forensic-qas-audit-9-1-11, DNA Advisory Board, Federal Bureau of Investigation, US Department of Justice.
  8. Quality Assurance Standards for Convicted Offender DNA Databasing Laboratories. http://www.fbi.gov/about-us/lab/biometric-analysis/codis/database-qas-audit-9-1-11, DNA Advisory Board, Federal Bureau of Investigation, US Department of Justice.
  9. Quality Assurance Standards for Forensic DNA Testing Laboratories and for Convicted Offender DNA Databasing Laboratories, http://www.fbi.gov/hq/lab/fsc/backissu/july2000/codispre.htm, Forensic Science Communications, July 2000   Volume 2   Number 3
  10. National Research Council report.  (1992). DNA Technology in Forensic Science.
    • Chapter 1. Introduction
    • Chapter 2. DNA Typing: Technical Considerations
  11. http://www.fbi.gov/about-us/lab/biometric-analysis/codis/swgdam-interpretation-guidelines, Forensic Science Communications, October 2001, Vol 3, No. 4.
  12. Genomic DNA Purification from Cigarette Butts and Buccal Swabs, Promega Application Note 11
  13. Inhibition and Facilitation of Nucleic Acid Amplification, Ian G. Wilson, Applied and Environmental Microbiology, Oct 1997, p. 3741–3751 Vol. 63, No. 1012
  14. Evaluation of an Alkaline Lysis Method for the Extraction of DNA from Whole Blood and Forensic Stains for STR Analysis, Klintschar M, Neuhuber F.,  J Forensic Sci 2000;45(3): 669–673.
  15. Overview and History of DNA Typing
  16. Evaluation of three methods for effective extraction of DNA from human hair, Emi Suenaga, Hiroshi Nakamura, Journal of Chromatography B, 820 (2005) 137–141.
  17. Expedited, Chemically Enhanced Sperm Cell Recovery from Cotton Swabs for Rape Kit Analysis Jessica V. Norris,1 M.Sc.; Kate Manning,1 Bsc.; Sarah J. Linke,1 B.S.; Jerome P. Ferrance,1 Ph.D.; and James P. Landers,1,2 Ph.D., J Forensic Sci, July 2007, Vol. 52, No. 4
  18. Extraction of DNA from decomposed human tissue An evaluation of five extraction methods for short tandem repeat typing, Per Hoff-Olsena ,*, Bente Mevaga, Eva Staalstrøma, Bente Hovdea, Thore Egeland, Bjørnar Olaisen, Forensic Science International 105 (1999) 171–183
  19. Increasing DNA extraction yield from saliva stains with a modified Chelex method, David Sweet, Miguel Lorente, Aurora Valenzula, Jose Lorente, J. Carlos Alvarez, Forensic Science International, 83 (1996), 167-177.
  20. Removal of a PCR Inhibitor and Resolution of DNA STR Types in Mixed Human-Canine Stains from a Five Year Old Case, Gary G. Shutler Ph.D.; Pierre Gagnon, Dipl.Med.L.T.; Gary Verret, Howard Kalyn,4 B.S.(HONS); Sandra Korkosh M.S.; Eric Johnston M.S.; and Joy Halverson D.V.M., J Forensic Sci 1999;44(3):623–626.
  21. NaOH Treatment to Neutralize Inhibitors of Taq Polymerase,  Michael T. Bourke Ph.D.; Carol A. Scherczinger Ph.D.; Carll Ladd Ph.D.;and Henry C. Lee Ph.D.,  J Forensic Sci 1999;44(5):1046–1050.
  22. AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples,  Jason Y. Liu, Ph.D.; Chang Zhong, Ph.D.; Allison Holt, Ph.D.; Robert Lagace, B.S.; Michael Harrold, M.S.; Alan B. Dixon, M.S.F.S.; Maxim G. Brevnov, Ph.D.; Jaiprakash G. Shewale, Ph.D.; and Lori K. Hennessy, Ph.D.  J Forensic Sci 2012;57(5):1022–1030.
  23. Developmental Validation of the PrepFiler™ Forensic DNA Extraction Kit for Extraction of Genomic DNA from Biological Samples,  Maxim G. Brevnov, Ph.D.; Hemant S. Pawar, Ph.D.; Janna Mundt, Ph.D.; Lisa M. Calandro, M.P.H.; Manohar R. Furtado, Ph.D.; and Jaiprakash G. Shewale, Ph.D.,  J Forensic Sci 2009;54(3):599–1607.