Day 1
Welcome and introductions
8:30 a.m.–9:15 a.m.
- Purpose of the workshop
- Instructors
- Facilities
- Safety and laboratory protocols
- Agenda review
- Brief overview of laser microdissection (LM) and fluorescent in situ hybridization (FISH) processes
Lecture: Introduction to LM (Group 1)
9:15 a.m.–10:15 a.m.
- Applications
- Mixtures of cells with same morphology
- Mixtures of cells with different morphology
- Instrumentation
- Laser microdissection (LM)
- Laser capture microdissection (LCM)
In-Lab Training (Group 2)
9:15 a.m.–10:15 a.m.
- FISH slide preparation
- Cell mixture slide preparation
Break
Lecture: Introduction to FISH (Group 1)
10:30 a.m.–12:30 p.m.
- General overview
- Procedure
- CEP probes
- Downstream processing
- Laser microdissection
- Extraction
- Amplification
- Applications
- Advantages / limitations
In-Lab Training (Group 2)
10:30 a.m.–12:30 p.m.
- FISH pretreatment
- FISH hybridization
- Introduction to the Carl Zeiss PALM System
Lunch
In-Lab Training (Group 1)
1:30 p.m.–2:30 p.m.
- FISH slide preparation
- Cell mixture slide preparation
Lecture: Introduction to LM (Group 2)
1:30 p.m.–2:30 p.m.
- Applications
- Mixtures of cells with same morphology
- Mixtures of cells with different morphology
- Instrumentation
- Laser microdissection (LM)
- Laser capture microdissection (LCM)
Break
In-Lab Training (Group 1)
2:45 p.m.–4:45 p.m.
- FISH pretreatment
- FISH hybridization
- Introduction to the Carl Zeiss PALM System
Lecture: Introduction to FISH (Group 2)
2:45 p.m.–4:45 p.m.
- General overview
- Procedure
- CEP probes
- Downstream processing
- Laser microdissection
- Extraction
- Amplification
- Applications
- Advantages / limitations