The Global Forensic and Justice Center at Florida International University is dedicated to supporting the justice community through innovative research, programs, evaluation of the latest technologies, forensic science education and laboratory quality reviews. Our goal is for all stakeholders to receive and provide the highest level of forensic science services.
As part of this commitment, we are making available the nearly 30-year archive of training material, symposia, poster presentations, and technical evaluations completed by our founding component, the National Forensic Science Technology Center (NFSTC).
Laser Microdissection Technology Transfer Workshop for Technical Managers and Supervisors
August 2-3, 2007
Workshop Overview - The purpose of this technology transfer workshop is to provide the student with an introduction to laser microdissection (LMD), methods for preparing samples for LMD, LMD operation, methods for DNA analysis of LMD recovered cells, and an understanding of the application of LMD recovery to sexual assault evidence analysis.
Sponsored by the National Institute of Justice, U.S. Department of Justice
Welcome and Introduction
Introduction to LMD technology
- Basic microscopy
- Components
- imaging
- Fluorescence
- DIC
- Laser dissection
- Basic theory
- History of LMD microscopy
- Other LMD technologies and tools
Basic cell identification: sperm and epithelial cells
LMD dissection of sperm cells from a mixture
Lab Practical Overview
- LMD LAB - Care and Basics of LMD 6000
- LMD LAB - Demo of Leica LMD 6000: Using the microscope
- LMD LAB - LMD exercises - Recovery of cell samples
- WET/CHEM LAB - Fluorescence Sample Prep
- LMD LAB - LMD exercises - Recovery of cell samples
- Review of protocols-
LMD Research Studies from RFUMS
Presentation | Video 1 | Video 2
- Technical Obstacles
- Histology Study
- DNA Isolation Study
- DNA Yield Study
- Mixture Study
- Low Copy Number Study
- Comparative Study with Preferential Lysis
- Case Studies
Advanced LMD Forensic Applications
- Fluorescent Sperm Identification
- Chromosome Paint for Diploid Cells
- Validation Issues
- LMD on Sexual Assault Kits
LMD LAB
- Fluorescence
- AVC Software Module
- Advanced Operations
LMD Microscopy Research and Experience from NLCL
- Why LMD for NLCL Casework
- Elimination of Manual Extraction
- Reproducibility
- Sensitivity
- Troubleshooting
- Single Amplification/Optimization
- Absence of Sperm
- LMD: Streamlined, Novel Process
- Casework Examples
Research on Chromosome Paint of Diploid Cells
- Cell Preparation for Chromosome Paint
- Male/Female Diploid Cells Mixtures
- Non-probative case results
Novel Processing Plan for the Implementation of LMD Analysis of Sexual Assault
Review DNA Data from LMD samples
- Admissibility / voir dire issues
- QA & accreditation
- Competency training
- Harmonious implementation
- Commitments within the forensic community
Global Forensic and Justice Center
11200 SW 8th Street
Miami, FL 33199