Laser Microdissection Workshop for Technical Managers && Supervisors Agenda – Day 1
Time
Topic
Instructor(s)
8:30 – 9:00 a.m. Welcome and Introduction
  1. Instructors
  2. Students
  3. Facilities
  4. Agenda Review

Watch Video		 Sanders, Wojtkiewicz,
Raley
9:00 – 10:00 a.m. Introduction to LMD technology
  1. Basic microscopy
    1. Components
    2. imaging
    3. Fluorescence
    4. DIC
  2. Laser dissection
    1. Basic theory
    2. History of LMD microscopy
    3. Other LMD technologies and tools
 Wojtkiewicz
10:00 – 10:15 a.m. Break  
10:15 – 10:30 a.m. Basic cell identification: sperm and epithelial cells

Watch Video		 Raley
10:30 – 10:45 a.m. LMD dissection of sperm cells from a mixture

Watch Video		 Sanders
10:45 – 11:00 a.m. Lab Practical Overview

Watch Video		 Sanders
11:00 – 11:15 a.m. LMD LAB – Care and Basics of LMD 6000 Lee
11:15 a.m. – 12:00 p.m. LMD LAB - Demo of Leica LMD 6000: Using the microscope Sanders
Raley
12:00 – 1:00 p.m. Lunch  
1:00 – 2:00 p.m. LMD LAB - LMD exercises – Recovery of cell samples
Groups 1 && 2 		Sanders
Raley
  WET/CHEM LAB - Fluorescence Sample Prep. - Groups
3 && 4		 Wojtkiewicz
1:00 – 2:00 p.m. LMD LAB - LMD exercises – Recovery of cell samples
Groups 3 && 4		 Sanders
Raley
  WET/CHEM LAB - Fluorescence Sample Prep. - Groups
1 && 2		 Wojtkiewicz
3:00 – 3:15 p.m. Break  
3:15 – 3:30 p.m. Review of protocols performed by NFSTC Staff Sanders
3:30 – 4:20 p.m. LMD Research Studies from RFUMS
  1. Technical Obstacles
  2. Histology Study
  3. DNA Isolation Study
  4. DNA Yield Study

Watch Video		 Sanders
4:20 – 4:30 p.m. Q&A  

Go to Day 2

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