Mitochondrial and STR DNA Analysis by Mass Spectrometry Workshop Outline – Day 1 |
Time |
Topic |
Instructor(s) |
| 8:30–8:45 | Welcome & Introduction
| NIJ Staff NFSTC Staff Hofstadler, Lowery, Hall, Planz, McCurdy, Budowle |
| 8:45–9:45 | Introduction to Biological Mass Spectrometry
|
Hofstadler |
| 9:45–10:15 | High Throughput Analysis of Amplified Nucleic Acids with Mass Spectrometry: The Ibis Platform
|
Hofstadler |
| 10:15–10:30 | Break | |
| 10:30–11:00 | Introduction to Mitochondrial DNA Analysis | McCurdy |
| 11:00–11:30 | Overview of the Ibis mtDNA Tiling Assay
|
Hall |
| 11:30–12:15 | Mitochondrial DNA Mixture Detection, Analysis, and Interpretation
|
McCurdy |
| 12:15–1:15 | Lunch | |
| 1:15–1:45 | Overview of the Ibis STR Assay
|
Hall |
| 1:45–2:30 | Enhanced Resolution and Statistical Power Through SNP Distribution Within the Short Tandem Repeats
|
Hall |
| 2:30–2:45 | Break | |
| 2:45–3:15 | Analysis Software for the mtDNA Tiling Assay Overview
|
Hall |
| 3:15–3:45 | Laboratory Tour | Lowery, Hofstadler |
| 3:45–4:00 | Ibis Assay System Workflow Overview
|
Lowery |
| 4:00–4:30 | Laboratory Exercise Overview
|
Lowery |
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