Dart Mass Spectrometry Workshop Agenda – Day 1

1. How DART works
2. TOF basics
a. Overview of kinds of information acquired

Presentation

1. How to collect a spectrum
2. Calibration
3. Measuring an exact mass
4. Getting an elemental composition

1. Method
   1. Sample preparation
   2. Function switching for CID spectra at four orifice voltages
   3. Calibration: PEG600 and using a lock mass with check standards
   4. SearchFromList program: searching empirical formula and drug spectral libraries
   5. Advantages: four spectra in one second, more peaks = more spectral information = possible ID reproducible spectra
   6. Disadvantages: less sensitivity, “mixture” spectra (no prior chromatography), ‘finite’ database for searching
2. Application
   1. Screening technique, so far
      1. Drugs in any dosage form
      2. GHB screening method
   2. Method Validation at VaDFS
   3. Possibility as an ID technique in near future
   4. Example data

Presentation

1. Set up and demonstrate a function switching method – tune files and method file
2. Demonstrate running a sample – calibration standard/lock mass/sample
3. Demonstrate SearchFromList for drug spectra at all four orifice voltages
4. Students run unknowns of solid dosage forms Steiner